extraction plant nitrogen

A method for extracting high-quality total RNA from …

Acquiring high quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations. At present, a large number of nucleotide isolation methods have been …Web

A comprehensive comparison of four methods for extracting lipids …

The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific functions in plant development, growth and stress responses have yet to be fully elucidated. To do this, efficient lipid extraction protocols which deliver target compounds in solution at concentrations adequate for subsequent detection, …Web

A Review of Conventional and Emerging Process …

Darwin has the only helium extraction plant in the southern hemisphere (West 2009). However, as the projections in Figure 1 show, the production capacity of these plants and other planned projects currently fail to meet the projected demand for helium. *Author to whom all correspondence should be addressed. E-mail: [email protected] (E. May).Web

Root sampling and RNA extraction methods for field-based gene

The root samples were ground using a mortar and pestle in liquid nitrogen and stored at −80°C until RNA extraction. For the extraction of total RNA from the root samples, we used the RNeasy Plant Mini Kit (Qiagen, The Netherlands) alone or in combination with Fruit-mate for RNA Purification (Takara Bio Inc., Japan) and RNAiso Plus (Takara ...Web

Comparison of three genomic DNA extraction methods to obtain …

The extraction of high-quality DNA from plant tissue is time consuming, arduous, and costly due to multiple steps and the high cost of liquid nitrogen. In addition, the problems associated with the available commercial kits are their high cost and low yield of DNA [5, 7]. Several methods to isolate DNA from plant tissues are available; however ...Web

A single-step method for RNA isolation from tropical crops in

The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction ...Web

Protocol: a fast, comprehensive and reproducible one-step extraction

Overview of the experimental workflow for the MTBE-based extraction procedure. Plant material is harvested and snap-frozen in liquid nitrogen. The harvested tissue is homogenized using a pre-cooled mortar and pestle or cooled tubes in a mixer mill. About 10–50 mg ±10% of the frozen powder is weighed in pre-labelled 2 ml …Web

Protocol: a simple method for extracting next

The majority of DNA extraction methods from plant leaf tissue are derived from the original hexadecyltrimethylammonium bromide (CTAB) based method, …Web

A high throughput DNA extraction method with high yield

The use of fresh tissue and liquid nitrogen cooled plates reduce the throughput. The claimed DNA yield from the instruction manual is 1 to 15 μg DNA per sample. ... A Rapid Method for High Throughput DNA Extraction from Plant Material for PCR Amplification. Plant Mol Biol Rep. 2000, 18: 61-64. 10.1007/BF02825295. Article …Web

IOP Conference Series: Materials Science and Engineering …

LNG plant is operated as a base-load plant without any helium extraction unit before, now a helium extraction and nitrogen removal unit (HeX-NRU) is being considered to be integrated in this plant as shown in Figure 2. Table 1 Composition of Helium-bearing BOG from LNG storage tank Component BOG （vol %） Methane 88.799 Ethane 6.573×10-3Web

A simple and efficient genomic DNA extraction protocol for large …

1. Introduction. Plants produce secondary metabolites that interfere not only with extraction of high quality genomic DNA but also with the subsequent reactions such as PCR and related genetic analyses (Kotchoni and Gachomo, 2009, Kotchoni et al., 2011).The widely used genomic DNA extraction procedures rely on lengthy protocols that use …Web

A simple plant high-molecular-weight DNA extraction method …

Here, we present a simple, nuclei-CTAB method for the extraction of large, in-tact HMW gDNA from plants. This procedure is simple, economical, rapid, and offers …Web

Leaf Tissue Sampling and DNA Extraction Protocols

The ratio of leaf tissue to silica gel should be at least 1:5, preferably 1:10. Check the Ziploc bags at a regular interval, and replace the silica gel when its color changes from blue to pink. Keep replacing the silica gel until the blue color remains stable. Most leaf samples completely dry within 24 h. 3.Web

Horticulturae | Free Full-Text | Effects of Neem Leaf Extract on the

While lettuce offers essential human nutrients, it also contains anti-nutrients, particularly nitrate (NO3−). The use of neem leaf extract as a natural nitrification inhibitor has proven itself promising to remediate lettuce tissue NO3− content. This study evaluated the effects of neem leaf extract on soil properties, soil nitrification, lettuce growth, yield, …Web

Methods for Measuring Nitrate Reductase, Nitrite Levels, and …

3.1 Nitrate Reductase Activity. NR activity can also be performed from tungstate (200–500 μM) grown plants to check the active involvement of NR (Fig. 1a). 3.1.1 Preparation of Extract. 1. Excise the plant tissues with scissors and immediately shock freeze grind in liquid nitrogen into a fine powder (1 gFW).Web

Systems Biology for Enhanced Plant Nitrogen Nutrition | Science

Nitrogen (N) is the mineral nutrient required in the greatest amount by plants ( 1 ), and its availability, or lack thereof, limits plant growth and development ( 2 ), crop yield ( 3 ), and primary production at a planetary scale ( 4 ). Biologically available N forms, such as nitrate or ammonia, are in short supply in natural as well as ...Web

Simple extraction methods that prevent the artifactual conversion …

This hypothesis explains the differential effects of DMF on chlorophyllide formation during chlorophyll extraction from different plant species ... Increases of chlorophyll a/b ratios during acclimation of tropical woody seedlings to nitrogen limitation and high light. Plant Cell Environ. 2003, 26: 857-865. 10.1046/j.1365-3040.2003.01017.x. ...Web

What is the good method of protein extraction for plant leaves?

Well, when I worked on protein expression on N. benthamiana plants (tobacco) I evaluated two protein extraction methods: grinding using liquid nitrogen and employing a Tissue Lyser.Web

Comparison of laboratory methodologies to determine soil nitrogen

Chemical extraction . Chemical extraction (N q) was carried out adding 50 mL of a 2 M KCl (1:2.5; w:v) solution to 20 g soil in a stopped tube block digester at 100 °C for 4 h as described by Gianello and Bremmer (1986). After extraction, the concentrations of NO 3 –-N and NH 4 +-N ions were measured at room temperature.Web

Extraction Techniques for Plant-Based Bio-active Compounds

18.2 Classical or Conventional Extraction Methods. Classical extraction methods are basic extraction methods used to extract bio-active compounds from different types of plants. There are several classical methods including Soxhlet extraction, maceration, solvent extraction, hydrolization, infusion, and percolation.Web

Membrane Technologies for Nitrogen Recovery from Waste …

The data extraction was based on a search performed on the Web of Science on 05/10/2022 using the ... Digester reject water seems to be the best line for nitrogen recovery in wastewater treatment plants due to its high nitrogen content. Since this line is recycled back to the treatment train, studies that alter the physicochemical …Web

EP2971025A1

A method for extraction of nitrogen from organic materials through ammonification by mixed bacterial populations is provided. An organic material can be any protein-rich organic material e.g., of animal or plant origin. In order to more clearly appreciate the invention, the following terms are defined.Web

Plant Extraction Methods | SpringerLink

This step is conducted outside the percolator. It usually requires 4–5 h. Soaking: The swollen raw material is now placed inside the percolator, filled up with the remaining volume of solvent, and left undisturbed. This step lasts for 1–2 days, and extraction efficiency is similar to maceration.Web

Extraction of Plant Secondary Metabolites | SpringerLink

Online ISBN 978-1-61779-624-1. eBook Packages Springer Protocols. This chapter presents an overview of the preparation of extracts from plants using organic solvents, with emphasis on common problems encountered and methods for their reduction or elimination. In addition to generally applicable extraction protocols, methods are...Web

Extraction and analysis of nitrogen, phosphorus and carbon …

DOI: 10.1080/063161 Corpus ID: 96002691; Extraction and analysis of nitrogen, phosphorus and carbon fractions in plant material @article{Kedrowski1983ExtractionAA, title={Extraction and analysis of nitrogen, phosphorus and carbon fractions in plant material}, author={Richard A. Kedrowski}, …Web

Sample Collection and Storage | SpringerLink

Of importance to the successful extraction of genomic DNA from plant tissues is the collection of the suitable material and proper storage of the tissues before DNA isolation. If the samples are not properly treated, DNA can be degraded prior to isolation. ... While collection of tissues in liquid nitrogen and −80 °C storage may be highly ...Web

Leaf Tissue Sampling and DNA Extraction Protocols

1 Introduction The availability of direct polymerase chain reaction (PCR) system helps to amplify DNA in a few easy steps that eliminate the need for standard …Web

Extraction of High-Value Chemicals from Plants for Technical and …

Plants produce a variety of high-value chemicals (e.g., secondary metabolites) which have a plethora of biological activities, which may be utilised in many facets of industry (e.g., agrisciences, cosmetics, drugs, neutraceuticals, products, etc.). Exposure to various different environments, as well as their treatment (e.g., …Web

Fatal Poultry Plant Gas Release Linked to Poor Safety Procedures

The US Chemical Safety and Hazard Investigation Board determined the cause of the Jan. 21, 2021, nitrogen release at a Foundation Food Group plant in Gainesville, Ga., was from the failure of a freezer's control system to accurately measure and limit the …Web

DNA Extraction Protocol for Plants with High Levels of Secondary

Apart from traditional extraction approaches, several commercial kits are also accessible to extract genomic DNA from plants with sufficient quality . For extracting genomic DNA an initial mechanical grinding of the leaf sample is carried out in the presence of liquid nitrogen, where the ultimate aim is to access the nuclear material without ...Web