Ionizing Radiation and Complex DNA Damage: From Prediction …
60 Co γ-rays (LET=0.3 keV/μm), accelerated 11 B (E = 8.1 MeV/nucleon, LET=138 keV/μm) ions: It has been found that heavy charged particles induce clustered DNA damage in the genome of cells that can lead not only to gene mutations, but also to large deletions. ... Cyto- and chemokine response release by tumor cells after …Web
Cesium Irradiator < Yale Cancer Center
The Mark I Cesium-137 irradiator is designed principally for biomedical research. Its purpose is to provide accurate, convenient and reliable access to ionizing radiation to Yale University investigators for irradiations of molecular compounds, cell culture, animal and human tumor cells, and whole animal experiments. It serves as a useful ...Web
Abstract
Retrospective Comparison between 12-Gray and 8-Gray Total Body Irradiation (TBI) before Allogeneic Hematopoietic Cell Transplantation in Patients with …Web
Stem Cells and Irradiation
On the one hand, radiosensitivity/radioresistance of cancer stem cells affects the radiocurability of tumors, on the other hand, radiosensitivity is responsible for the …Web
An in vitro cell irradiation protocol for testing …
Traditionally, ultraviolet light (100–400 nm) is considered an exogenous carcinogen while visible light (400–780 nm) is deemed harmless. In this work, a LED irradiation system for in vitro photocytotoxicity testing is described. The LED irradiation system was developed for testing photopharmaceutical drugs, but was used here to …Web
Cancers | Free Full-Text | The Radiobiological Effects of Proton
In contrast, there was no dramatic difference in cell-cycle distribution of Chinese hamster ovary cells when comparing the response to low-LET 138 MeV PBT and 200 keV X-rays, where a degree of G 2 /M accumulation (~1.2–2-fold increase) of cells irradiated at 5 Gy dose equivalent, particularly at 6–12 h following both irradiation types, …Web
Frontiers | Ionizing Radiation-Induced Immune and Inflammatory
Recently, it has been shown that senescence is another major cell death mechanism developing at a later time point in the surviving endothelial cells. Irradiation doses in the range of 2–8 Gy led to increased DNA damage and a reduced repair efficiency in rat primary cerebrovascular endothelial cells, which were accompanied by increased …Web
Cellular and Biochemical Effects of Combined X-Ray Radiation …
When irradiation studies are performed in vivo, 18 interpretation of changes in peripheral blood cells is difficult due to multiple and complex actions occurring to other structures interacting with blood cells. Although limited in scope, ex vivo studies using irradiated whole blood, as in the current work, allow separating the direct effects ...Web
324 VOL 210
cells, this quantitative difference is dramatic; radiation doses of tho order of thousands rads delivered to the carbohydrate-containing part of the growth medium wereWeb
Immune recognition of irradiated cancer cells
Introduction. For nearly a century, the antineoplastic effects of radiation therapy (RT) have been catalogued as a purely cancer cell-intrinsic phenomenon. 1 Indeed, the elevated …Web
Determination of cell survival after irradiation via clonogenic assay
In Figure 2a and 2b the survival data for A 549 cells of clonogenic and multiple MTT assay are plotted against irradiation dose. The correlation of both assays is presented in Figure 2c and 2d (R 2 = 0.99 for both). As expected, the survival curves of both assays show the same characteristics: the survival depends on the dose of irradiation and an …Web
Bone marrow adipocytes promote the regeneration of stem cells …
After irradiation or chemotherapy, these cells are depleted while adipocytes become abundant. We discovered that bone marrow adipocytes synthesize SCF. They arise from Adipoq-Cre/ER + progenitors, which represent ∼5% of LepR + cells, and proliferate after irradiation. Scf deletion using Adipoq-Cre/ER inhibited haematopoietic regeneration ...Web
Frontiers | Local Irradiation Sensitized Tumors to Adoptive T Cell
Our findings provide evidence to support that local irradiation enhanced the therapeutic efficacy of adoptive T cell therapy for cancer, indicating that the combination …Web
Laser Irradiation of Organic Tattoo Pigments Releases …
Laser irradiation of P.Y.138 in postmortem tattooed pig skin mostly leads to the formation of xylene, benzene, and chlorinated benzenes ... 138 and the diazo pigment orange (P.O.) 13 in pig skin and their cyto- and genotoxic effects on human HaCaT and BJ skin cell lines. (a) Microscopic pictures of postmortem tattooed pig skin sections ...Web
Radiation-induced cell death mechanisms | Tumor Biology
Apoptosis is the major cell death modality observed in response to irradiation of cells naturally prone to apoptosis such as hematopoietic cells and their malignant counterparts . These cells are typically programmed for rapidly induced, rapidly executed, apoptotic death occurring within hours after exposure to radiation. ... 138], …Web
Frontiers | Modulating Both Tumor Cell Death and …
In this review we will present an overview of cell death modalities induced by IR, summarize the immunogenic properties of …Web
Laser Irradiation of Organic Tattoo Pigments Releases
Laser irradiation of P.Y.138 in postmortem tattooed pig skin ... Regarding skin cells, aniline only marginal increased sister chromatid exchanges in human skin fibroblasts (Wilmer et al., 1981). ...Web
The DNA damage response pathway regulates the expression of …
Majeti, R. et al. CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells. Cell 138, 286–299 (2009).Web
Molecular mechanisms of tumor resistance to radiotherapy
Cancer is the most prevalent cause of death globally, and radiotherapy is considered the standard of care for most solid tumors, including lung, breast, esophageal, and colorectal cancers and glioblastoma. Resistance to radiation can lead to local treatment failure and even cancer recurrence. In this review, we have extensively discussed …Web
Radiation-induced cell death mechanisms | Tumor Biology
In this review, apoptosis and mitotic catastrophe, the two major cell deaths induced by radiation, are described and dissected in terms of activating mechanisms. …Web
Dangerous Cell Phone Radiation Levels: What You Should Know
The Google Pixel 6, iPhone 13 Pro, and iPhone 12 (during this specific test) all measured at just around 1.00 watts per kilogram of radiation. Samsung's Galaxy S21 Ultra has just 0.71 watts per ...Web
Effects of different doses of X‑ray irradiation on cell apoptosis, cell
The present study examined the radiation biological response of cancer cells to different fractional irradiation doses and investigates the optimal fractional irradiation dose with improved biological effects. Radiobiological studies were performed at the molecular and cellular levels to provide insights into DNA damage and repair, and the apoptosis …Web
Radiation-induced cell cycle perturbations: a computational tool
Giorgio Baiocco Scientific Reports 11, Article number: 925 ( 2021 ) Cite this article 5284 Accesses 14 Citations 2 Altmetric Metrics Abstract Cell cycle progression …Web
Exosomes Derived From Adipose-Derived Mesenchymal Stem Cells …
ObjectiveStudies have shown that the therapeutic effects of mesenchymal stem cells (MSCs) are mediated in a paracrine manner, mainly through extracellular vesicles such as exosomes. Here, we designed a study to investigate whether exosomes derived from adipose-derived mesenchymal stem cells (ADMSC-Exos) had protective effects in a rat …Web
Current Status and Perspectives of Irradiation-Based Conditioning
1. INTRODUCTION. Allogeneic hematopoietic stem cell transplantation (allo-HCT) is the treatment of choice for many hematologic conditions. Acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) are the most common indications for allo-HCT [1,2].Transplantation must be preceded by administration of a conditioning regimen …Web
The radioresistant and survival mechanisms of
2. Cell structure and gene composition2.1. Unique cell wall. D. radiodurans is an aerobic gram-positive bacterium with a pink color and an optimal growth temperature of 30 °C. Under normal growth conditions, D. radiodurans in the logarithmic growth phase exists in the form of doublets, while D. radiodurans in the stable phase mainly exists in …Web
Radiation-induced signaling pathways that promote cancer cell …
Irradiation induces G1 and G2 cell cycle checkpoint activation and DNA repair. Most cancer cells are defective in G1 checkpoint, commonly due to the mutations/alterations of the key regulators of the G1 checkpoint (in blue), but contain a functional G2 checkpoint. ... Radiat Res. 1994; 138:S85–S88. [Google Scholar] 98. …Web
Radiation-induced cell cycle perturbations: a computational tool
Cell-cycle characterization. After irradiation, cells were incubated with (2, mu g/ mu l) EdU (Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit, Invitrogen, USA) for 1 h, then ...Web
Radiation Oncology/SCLC/PCI
RTOG Abstract, 1996 -- "A randomized phase III study of prophylactic cranial irradiation (PCI) vs. observation (OBS) in patients (Pts) with small cell lung cancer (SCLC) achieving a complete response: Final report of an incomplete trial by the Eastern Cooperative Oncology Group and Radiation Therapy Oncology Group."Web
Multifunctional theranostic nanoplatform loaded with autophagy
Volume 138, July 2022, 212919. ... Upon CQ loading, PIFC NPs induced remarkable cell death, and under the same NIR irradiation, 80% cell death was observed within 24 h (Fig. 4 a). What's more, PIF NPs and PIFC NPs solutions at various levels (0, 150, 300, 450, 600 and 750 μg/mL) were administered to cells submitted to irradiation at different ...Web
