Detection of the residual concentration of sodium dodecyl …
Results: High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.
(PDF) The Efficiency of Decellularization of Bovine …
The aim of the work is evaluation of efficiency of bovine pericardial decellularization, based on the use of trypsin enzyme with 1% Sodium Dodecyl Sulfate (SDS) and 0.1% SDS. Methods.
Tissue extraction of DNA and RNA and analysis by the
For paraffin wax embedded tissue a five day incubation with proteinase K was required to produce good yields of DNA. Incubation with sodium dodecyl sulphate produced very poor yields, while boiling produced 20% as much DNA as long enzyme digestion. DNA extracted by these methods was suitable for the PCR amplification of a single copy gene.
Residual sodium dodecyl sulfate in decellularized muscle
Detergents such as sodium dodecyl sulfate (SDS) are commonly used to extract cells from tissues in a process called "decellularization". Residual SDS is difficult to completely remove and may lead to an undesirable host response towards an implanted biomaterial. In this study, we developed a modific …
Nano-hydroxyapatite: A Driving Force for Bone Tissue …
Bone is an interesting nanocomposite tissue, comprising organic (primarily collagen) as well as inorganic (primarily nano-hydroxyapatite [n-HA]) constituents. ... Shimabayashi et al. functionalized the surface of HA with the amphiphilic surfactant sodium dodecyl sulfate. Inorganic materials can also be used as suitable surface modifiers. For ...
Decellularization of Full Heart—Optimizing the Classical Sodium-Dodecyl …
Decellularization with sodium dodecyl sulfate was found to be more suitable and resulted in scaffolds with a low amount of DNA, a highly preserved extracellular matrix composition, and structure shown by GAG quantification and immunohistochemistry. ... Static cultured tissues were fixed in 4% PFA for 6–8 h on a shaker at room temperature ...
Application of Detergents or High Hydrostatic …
Uterine tissues were extracted from Sprague Dawley (SD) rats and decellularized using either sodium dodecyl sulfate (SDS) or high hydrostatic pressure (HHP) at optimized conditions. Histological staining …
Post-decellularization techniques ameliorate cartilage
Extracellular matrix (ECM) is the non-cellular component present within all tissues and organs. It provides the essential physical scaffold for the cellular constituents and initiates crucial biochemical and biomechanical signals that are required for tissue morphogenesis, differentiation and homeostasis. 1 Cartilage is a hyalin and an avascular …
Cardiac-derived extracellular matrix: A decellularization
Extracellular matrix (ECM) is a fundamental component of the heart, guiding vital cellular processes during organ homeostasis. Most cardiovascular diseases lead to a remarkable remodeling of the ECM, accompanied by the formation of a fibrotic tissue that heavily compromises the heart function. Effective therapies for managing fibrosis and …
Sodium Dodecyl Sulfate
Sodium dodecyl sulfate. SDS, also known as lauryl sulfate, is an ionic detergent that is useful for the rapid disruption of biological membranes as well as the breakdown of tissue architecture. Concurrently, SDS is a powerful inhibitor of both RNase and DNase activity. SDS is commonly prepared as either a 10% or a 20% (w/v) stock solution and ...
Sodium dodecyl sulphate: A very useful surfactant for …
Sodium dodecyl sulfate (SDS) is a key surfactant that is widely used in textile processing as a detergent depending upon its properties like low cost, high water solubility, and dirt removal ...
FACT or PACT: A Comparison between Free-Acrylamide and …
We systematically compared a number of factors including hydrogel formation, clearing solution, and clearing temperatures between free-acrylamide and acrylamide-based passive sodium dodecyl sulfate (SDS) tissue clearing and discussed negative effects of polyacrylamide on clearing, staining, and imaging in detail.
FACT or PACT: A Comparison between Free-Acrylamide and
We systematically compared a number of factors including hydrogel formation, clearing solution, and clearing temperatures between free-acrylamide and acrylamide-based passive sodium dodecyl sulfate (SDS) tissue clearing and discussed negative effects of polyacrylamide on clearing, staining, and imaging in detail.
Evaluation of two decellularization methods in the …
Results: The complete tissue decellularization was successfully achieved after treatment with sodium dodecyl sulphate (SDS) and Triton X-100. The total absence of nuclear structures and removal of viable cells were confirmed by haematoxylin-eosin staining and scanning electron microscopy.
Decellularization of Full Heart—Optimizing the Classical Sodium-Dodecyl …
Decellularization with sodium dodecyl sulfate was found to be more suitable and resulted in scaffolds with a low amount of DNA, a highly preserved extracellular matrix composition, and structure shown by GAG quantification and immunohistochemistry. ... For this purpose, decellularized native tissues are a preferable choice as they provide an in ...
Sodium dodecyl sulfate
Sodium dodecyl sulfate has been used: as an ionic surfactant in polystyrene (PS) particle suspension for surfactant-based experiments; in phosphate-buffered saline as a component of lysis buffer to homogenize myometrium and leiomyoma tissue; as a component of lysis buffer to dissolve formazans; as a component of high-salt nuclear extraction buffer
Matrix alteration and not residual sodium dodecyl sulfate
It has been suggested that residual cytotoxic sodium dodecyl sulfate (SDS) is responsible for the low levels of cell in-growth observed in SDS decellularized tissues. To determine whether this is the case, we used 2 washing methods to remove residual SDS and extensive biochemical, mechanical, and st …
Detection of the residual concentration of sodium …
Sodium dodecyl sulfate (SDS, formulation: CH3(CH2)11SO4Na), as a widely used ionic surfactant, (Schramm et al. 2003) is highly used in decellularization …
The impact of decellularization methods on extracellular
Tissue-derived decellularized biomaterials are ideal for tissue engineering applications as they mimic the biochemical composition of the native tissue. ... such as sodium dodecyl sulphate (SDS ...
62862, Sodium dodecyl sulfate, 151-21-3, Sigma-Aldrich
Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate (SLS), has an alkyl tail of 12 carbon atoms attached to a sulfate group. It is an anionic surfactant due to the negative charge of its sulfate group. ... Due to its ability to disrupt the tissue architecture, SDS is preferred in several nucleic acid purification reagents. SDS has ...
The impact of detergents on the tissue decellularization …
Biologic scaffolds are derived from mammalian tissues, which must be decellularized to remove cellular antigens that would otherwise incite an adverse immune response. ... (Sigma-Aldrich), 8mM CHAPS (Sigma-Aldrich), 4% sodium deoxycholate (Sigma-Aldrich), 1% sodium dodecyl sulfate (SDS) (Bio-Rad), 0.1% SDS (Bio-Rad) or …
Decellularization in Tissue Engineering and …
Sodium dodecyl sulfate (SDS), sodium deoxycholate (SD), and Triton X-200 are ionic detergents that have been used in decellularization (Hudson et al., 2004; Gilbert et al., 2006; Lumpkins et al., 2008; Montoya and McFetridge, 2009; Zhou et al., …
Tissue Scaffolds Derived from Rat Skin and Clinical …
Sodium dodecyl sulfate is more effective than Triton X-100 for removing nuclei from the dense tissue. Sodium dodecyl sulfate tends to disrupt the native tissue structure and causes a decrease in the GAG concentration and a loss of collagen integrity . Sodium deoxycholate sulfate is also an ionic detergent which is very effective for …
Decellularization
Sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), and Triton X-100 are the most widely applied detergents [77]. Surfactants solubilize cytoplasmic and nuclear lipid membranes. Compared with ex vivo tissues, milder and shorter decellularization times can be employed; however, the protein structure is affected unless tightly controlled.
Decellularization Strategies for Regenerative Medicine: …
The widely used ionic surfactant, sodium dodecyl sulfate (SDS), has been successful in a number of applications in decellularization because of its ability to efficiently remove cells and genetic material. ... To further increase porosity, the tissue also underwent sonication in sodium hydroxide. Creating a more porous scaffold was desired in ...
Immunogenicity of decellularized extracellular matrix …
Tissue-engineered decellularized extracellular matrix (ECM) scaffolds hold great potential to address the donor shortage as well as immunologic rejection attributed to cells in conventional tissue/organ transplantation. ... Friedrich EE, et al. Residual sodium dodecyl sulfate in decellularized muscle matrices leads to fibroblast activation in ...
Characterization of Porcine Urinary Bladder Matrix …
UBM sca ff olds, sodium dodecyl sulfate (SDS), which is a strong detergent, was used to try to tackle the issue of inadequate decellularization, with the concentration ranging from 0.1% up to 1% ...
Decellularization for the retention of tissue niches
tissue, regardless of the origin of the cells being seeded.4,5 Interestingly, it has been shown that ECM from various species can support human stem cell proliferation and dif- ... Triton X-100 and the ionic detergent Sodium Dodecyl Sulfate (SDS). Triton X-100 removes cellular content by
Journal of Forensic Research /A
Assessing Tissue Lysis with Sodium Dodecyl Sulphate for DNA Extraction from Frozen Animal Tissue Mobolaji Temitope Akinwole1 and Isaac Adeyemi Babarinde 2* 1Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria 2Department of Biology, Southern University of Science and Technology, Shenzhen, P.R. …
Decellularization of Skin Tissue | SpringerLink
Two anionic (sodium deoxycholate and sodium dodecyl sulphate) and two non-ionic biological detergents (tritron-×100 and tween-20) with trypsin were used for making acellular dermis matrix. The tissue matrix in each solution was continuously agitated at 180 rpm at 37 °C in orbital shaker to provide better contact of tissue with treatments ...
